Error Message:

Could not find any resources appropriate for the specified culture or the neutral culture.  Make sure "AjaxControlToolkit.Properties.Resources.NET4.resources" was correctly embedded or linked into assembly "AjaxControlToolkit" at compile time, or that all the satellite assemblies required are loadable and fully signed.

Reason:

AjaxControlToolkit’s control load reference refers to the base System.Web.UI.Control method which is present as a part of the ASP.NET AJAX Libraries and those libraries are referenced only when the ScriptManager is referenced in the page.

Solution:

Add ScriptManager in the page and this error would be resolved.

            <asp:ScriptManager ID="ScriptManager1" runat="server">
            </asp:ScriptManager>